Composite plasmids

Details Composite plasmids Composite plasmids

C - Chemistry, Metallurgy

12

N

195/1.15

C12N 15/00 (2006.01) C07H 21/04 (2006.01) C12N 15/76 (2006.01)

Patent

CA 1186253

ABSTRACT OF THE DISCLOSURE A composite plasmid selected from the group consisting of plasmid pMNl having a molecular weight of 10.0 megadaltons, plasmid pMN2 having a molecular weight of 7.6 megadaltons and plasmid pMNl' and pMN2' both having a molecular weight of 11.3 megadaltons. The composite plasmid is formed by inserting plasmid pSLl obtained from Streptomyces lavendulae into plasmid pCRl obtained from Escherichia coli and has a kanamycin resistant genetic marker and the following digestion characteristics to restriction enzymes: (i) pMNl and pMN2 have one cleavage site to each of the restriction enzymes EcoRI and SalI, and (ii) pMNl' and pMN2' have two cleavage sites to each of the restriction enzymes EcoRI and SalI.

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