C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/3.2, 150/15.
C12Q 1/48 (2006.01) C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) C12Q 1/50 (2006.01)
Patent
CA 1203152
-0- COMPOSITION, ANALYTICAL ELEMENT AND METHOD FOR THE QUANTIFICATION OF CREATINE KINASE Abstract of the Disclosure A method is described for the analytical determination of creatine kinase in an aqueous liquid, such as blood serum. This method comprises: (A) contacting, generally in the presence of an electron acceptor (e.g. oxygen), (1) a sample of the liquid to be analyzed, and (2) reagents which effect in the presence of a creatine kinase-containing liquid, an ordered sequence of reactions wherein: (a) creatine phosphate and adenosine diphosphate react in the presence of creatine kinase to form creatine and adenosine triphosphate; (b) glycerol is phosphorylated in the pres- ence of glycerol kinase and adenosine tri- phosphate to form L-.alpha.-glycerophosphate; and (c) L-.alpha.-glycerophosphate is oxidized in the presence of .alpha.-glycerophosphate oxidase to produce an optical density change; and (B) quantitatively detecting the rate of such change. This method can be practiced in solution or on "dry" analytical elements containing a plurality of zones.
428168
Esders Theodore W.
Findlay John B.
Lynn Shirley Y.
Schubert Richard M.
Eastman Kodak Company
Gowling Lafleur Henderson Llp
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