Composition, analytical element and method for the detection...

C - Chemistry – Metallurgy – 12 – Q

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150/16, 150/3

C12Q 1/04 (2006.01) C09B 23/02 (2006.01) C09B 23/10 (2006.01) C09B 23/14 (2006.01)

Patent

CA 1211349

-i- COMPOSITION, ANALYTICAL ELEMENT AND METHOD FOR THE DETECTION OF BACTERIA Abstract of the Disclosure A composition, an analytical element and method for the detection of bacteria in specimen samples, e.g. biological fluids, are disclosed. The composition optionally, but preferably, comprises a metabolizable substrate (e.g. glucose) and a benzin- dole dye which undergoes a detectable color change when incubated in admixture with a bacterial micro- organism. Useful dyes include particular benz[cd]- indole, benz[e]indole and benz[g]indole compounds. The described analytical element contains this com- position, preferably, in a spreading zone. Detection of bacteria can be accomplished by bringing the com- position or element into contact with a specimen sample. This invention is particularly useful in detection of significant bacteriuria.

435052

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