Composition and method for detecting cancer with technetium...

G - Physics – 01 – N

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G01N 33/534 (2006.01) A61K 39/395 (2006.01)

Patent

CA 1186221

ABSTRACT OF THE DISCLOSURE F(ab')2 or Fab fragments of antibodies to: a) human chorionic gonadOtropin (hCG), hCG alpha subunit, hCG beta sub- unit, or an hCG-like material; or b) other tumor specific or tumor associated molecules, to include carcinoembryonic antigen (CEA), alpha fetoprotein (AFP), human melanoma associated anti- gens, human sarcoma associated antigens or other antigens, are radiolabeled with tecnetium-99m (Tc-99m). When the F(ab')2 or Fab fragments of antibody to such tumor associated antigens are injected intravenously into a patient, the radiolabeled compo- sition accumulates at tumor sites. The accumulation of the cancer seeking radiopharmaceutical at tumor sites permits detection by external gamma scintigraphy. Thus, the composition is useful in the monitoring, localization and detection of cancer in the body. In an alternative composition, a double antibody approach to tumor localization using radiolabeled F(ab')2 or Fab fragments is utilized. In this approach, a tumor specific antibody in the form of IgG, F(ab')2 or Fab is first administered to a patient intravenously. Following a sufficient period of time, a second antibody in the form of F(ab')2 or Fab is administered. The second antibody is radiolabeled with Tc-99m and has the property that it is reactive with the first antibody. This double antibody method has the advantage over a single antibody approach in that smaller tumors can be localized and detected and that the total amount of radioactive trace localized at the cancer site is increased.

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