Composition and method for isolation and purification of...

C - Chemistry – Metallurgy – 07 – K

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C07K 17/10 (2006.01) C07K 1/22 (2006.01) C07K 16/00 (2006.01)

Patent

CA 2057087

Abstract of the Disclosure Preparation of a composite of mannan binding protein attached to an insoluble, support matrix is accomplished by reacting cyanogen bromide activated beaded agarose with a buf- fered solution of mannan binding protein isolated from rabbit serum. The composite has utility as an affinity sorbent for IgM when divalent metal ions are incorporated in the bind- ing buffer. The composite does not show cross-reactivity (binding) with immunoglobulins of the G class.

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