Composition and method for modulating plant transformation

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/87 (2006.01) A01G 7/00 (2006.01) C12N 5/04 (2006.01) C12Q 1/68 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2697851

A plant culture medium composition for modulating plant transformation events, comprising a plant culture medium and an effective amount of at least one compound having a chloride component intermixed thereinto. In one embodiment, the at least one chloride-containing compound is selected from the group comprising: NaCl, MgCl2, and KCl. Another embodiment relates to a method for modulating the frequency of plant transformation events. The method comprises the steps of providing a plant culture medium composition and contacting at least one plant with the plant culture medium composition. At least one cell from the at least one plant is transformed with a nucleic acid of interest. The presence of at least one transformation event is detected and quantified. The frequency of quantified transformation events is compared with a suitable control. Changes in quantified transformations events compared to the control are indicative of changes in the frequency of plant transformation events.

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