Composition and method of assaying for trace amounts of...

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G01N 33/68 (2006.01) G01N 33/52 (2006.01)

Patent

CA 1333251

A new and improved method and composi- tion for determining the presence and concentra- tion of low to trace amounts of proteins, such as albumin, in a test sample, such as urine. The method includes using a reagent composition capable of interacting with low to trace amounts of proteins to produce a visually or instrument- ally detectable and/or measurable response. The method can be used in wet assays or in dry test strip assays, wherein the reagent composition is incorporating into a carrier matrix. The new and improved reagent composition, comprising a dye, such as a polyhydroxybenzenesulfonephthalein- type indicator, like pyrocatechol violet; a tung- state, such as ammonium tungstate; and, if neces- sary, a suitable buffer, is incorporated into the carrier matrix to provide sufficient sensi- tivity to low to trace protein levels and suffi- cient color resolution between low to trace pro- tein levels, thereby affording an accurate and trustworthy protein assay of test samples having a low protein concentration.

600070

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