G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 37/00 (2006.01) G01N 21/01 (2006.01)
Patent
CA 2356605
Process and formulation are described allowing fabrication of absorbance and fluorescence standards in cuvettes and micro-well plate, and other desirable containers, with particular application to drug discovery and high throughput screening of bioactive systems. The material medium is capable of incorporating a large number of dyes, individually or in combination, and can closely mimic real aqueous assays in optical properties such as the dye spectra, transparency, refractive index, and shapes of meniscus. The medium is compatible with addition of formulation components for control of foaming, vapor pressure, freezing point, dye bleaching, and molecular rotational correlation times. The process starts with the dispensing of a fluid dye- containing liquid into the vessel of choice, and its subsequent viscosification by chemical or physical means into a viscous gel. After further processing for stability, the container can be sealed with appropriate means. The standards are useful for calibration of spectrophotometric or fluorometric plate readers and imagers for correction of systematic spatial errors, for calibration of absolute intensities, and their replicates may be used to allow cross comparison of different instruments. Specialized standards can be used to check instrument performance in specialized assays relying on fluorescence resonance energy transfer (FRET), time-resolved fluorescence (TRF) and fluorescence polarization (FP).
Masoumi Zahra
Yekta Ahmad
Amersham Biosciences Niagara Inc.
Imaging Research Inc.
Robic
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