Composition for amplifying nucleic acids

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2584847

The invention relates to a concentrated and buffered liquid composition for amplifying nucleic acids, comprising at least one dNTP, at least one enzyme required for the amplification, at least one oligonucleotide primer, and at least one fluorescent nucleotide probe, in the presence of a polyol and/or of polyvinylpyrrolidone (PVP).

L~invention concerne une composition liquide concentrée et tamponnée destinée à amplifier des acides nucléiques, ladite composition comprenant au moins un dNTP, au moins une enzyme nécessaire à l~amplification, au moins une amorce oligonucléotidique et au moins une sonde nucléotidique fluorescente, en présence d~un polyol et/ou de polyvinylpyrrolidone (PVP).

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