G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 27/00 (2006.01) G01N 31/02 (2006.01) G01N 33/48 (2006.01)
Patent
CA 1041423
ABSTRACT OF THE DISCLOSURE A novel immunoassay technique is provided employing sacs having a lysable membrane and at least one determinant or epitopic site recognizable by an antibody. Enclosed in the sacs are water soluble stable free radical compounds, which are capable of escape from the sac upon lysis. The immunoassay can be employed for determination of an epitope containing compound, anti- bodies or complement. The basic modes are employed. The first mode depends upon the difference in the EPR spectrum of the stable free radical when enclosed in the sac, at relatively high localized concentra- tion, and when free in solution, at relatively low concentration. The amount of the free radical compound free in solution is dependent upon available antibody bound to the sacs and available complement. The second mode can be used for the determination of an epitope containing compound or antibody. In this mode, the antibody agglutinates the sacs, the agglutinated sacs are separated from the non-agglutinated sacs and either or both of the dif- ferentiated sacs lysed and the amount of free radical determined. The immunoassay is extremely sensitive and has a high multiplication factor, because a single event results in the free- ing of a large number of free radical molecules.
194607
Humphries Gillian M. K.
Mcconnell Harden M.
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