C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/40, 195/46
C12N 5/02 (2006.01)
Patent
CA 1177424
ABSTRACT A cell culture medium not relying on fetal bovine serum as a necessary component is disclosed. The medium optimally comprises from about 80-90% of a chemically defined nutrient medium and from about 10-20% of a natural biological fluid which has been treated by contact with "feeder" culture of smooth muscle cells for about 24 hours. The treatment may involve only natural fluid or such fluid in admixture with nutrient medium. Thymidine is then added in an amount such that its final concentration is between 10-4M and 10-6M. Such medium has been found suitable for the growth of mammalian cells, including mammalina endothelial cells. Additionally, lots of fetal bovine serum found unsuitable to support cell growth may be treated by contact with smooth muscle cells for about 24 hours and thereby converted to acceptable lots.
386521
Ryan James W.
Ryan Una S.
Sim & Mcburney
University Of Miami
LandOfFree
Composition for cell culture does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Composition for cell culture, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Composition for cell culture will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1236626