Composition for culturing multipotent stem cells and...

Details Composition for culturing multipotent stem cells and... Composition for culturing multipotent stem cells and...

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2504179

It is aimed to culture multipotent stem cells in a medium without using serum or supporting cells to thereby proliferate or establish undifferentiated multipotent stem cells sustaining the differentiation ability. This object can be achieved by preparing a medium, in which multipotent stem cells are to be cultured, exclusively using apparent components supplemented with an adenylate cyclase activity inhibitor.

La présente invention permet de mettre en culture des cellules souches pluripotentes dans un milieu sans utiliser ni sérum, ni cellules de support, afin de faire proliférer ou d'établir des cellules souches pluripotentes non différenciées conservant leur capacité de différenciation. A cet effet, on prépare un milieu, dans lequel les cellules souches pluripotentes seront mises en culture, en n'utilisant que des composants apparents complétés par un inhibiteur de l'activité de l'adénylate cyclase.

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