Composition for detecting beta-1,3-glucan, preparation...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/26 (2006.01) A61K 35/64 (2006.01) A61K 49/00 (2006.01)

Patent

CA 2397495

The present invention relates to a composition for detecting an infinitesimal quantity of beta-1,3-glucan, a preparation method thereof and a diagnostic kit detecting beta-1,3-glucan. The composition of the present invention shows phenoloxidase activity by beta-1,3-glucan in the presence of calcium ions. Using the composition of the present invention, a sample is gathered from a specimen, the composition of the present invention and calcium ions are added to the sample, and beta-1,3-glucan is detected by measuring phenoloxidase activity.

La présente invention concerne une composition qui permet de détecter une quantité infinitésimale de bêta-1,3-glucane dans un échantillon, un procédé de préparation de cette composition et une trousse de diagnostic utilisée pour détecter le bêta-1,3-glucane. La composition selon la présente invention présente une activité phénoloxydase par le bêta-1,3-glucane en présence d'ions calcium. Au moyen de la composition selon l'invention, un échantillon est obtenu à partir d'un prélèvement, la composition selon l'invention et des ions calcium sont ajoutés à l'échantillon et le bêta-1,3-glucane est détecté au moyen de la mesure de l'activité phénoloxydase.

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