G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/58 (2006.01) C12Q 1/68 (2006.01) G01N 21/78 (2006.01) G01N 33/533 (2006.01)
Patent
CA 2694030
Disclosed is a composition for measuring a binding affinity between a nucleic acid and a test substance, which contains an organic fluorescent substance capable of binding to an RNA and which emits fluorescence having an intensity greater while the organic fluorescent substance is liberated from an RNA than while the organic fluorescent substance is bound to an RNA. This enables a highly accurate and easy measurement of a binding affinity between a test substance and a nucleic acid, and allows various substances to be examined as a test substance.
Cette invention concerne une composition permettant de mesurer l'affinité de liaison entre un acide nucléique et une substance présentant un intérêt et contenant un phosphore organique capable de se lier à l'ARN et présentant une intensité de fluorescence plus élevée dans sa forme non liée à l'ARN par rapport l'intensité de fluorescence dans sa forme liée à l'ARN. Il est alors possible de mesurer l'affinité de liaison entre une substance présentant un interêt et un acide nucléique de manière précise et simple. Il est également possible d'utiliser diverses substances en tant que substance devant être testée.
Nakatani Kazuhiko
Sasaoka Shinichi
Umemoto Shiori
Wazaki Takahiro
Zhang Jinhua
Borden Ladner Gervais Llp
Nitto Kasei Co. Ltd.
Osaka University
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