Composition for stabilizing survival of transplanted...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/06 (2006.01) C07K 16/00 (2006.01) C12N 5/08 (2006.01) C12N 5/10 (2006.01) C12N 5/12 (2006.01) C12N 15/09 (2006.01) C12N 15/12 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2531569

[PROBLEMS] To stabilize the survival of transplanted hematopoietic stem cells by using HLA-matched activated lymphocytes. [MEANS FOR SOLVING PROBLEMS] HLA- matched activated lymphocytes in mononuclear cells separated from peripheral blood or umbilical cord blood are proliferated and activated. After separating and collecting, the HLA-matched activated lymphocytes are employed as the main component of a composition for stabilizing the survival of transplanted hematopoietic stem cells. The obtained composition is widely usable in, for example, prevention of insufficient survival of transplanted hematopoietic stem cells and therapy for promoting the survival thereof. Although the dose of the composition varies depending on the age, conditions, etc. of a patient, a humanized antibody is administered in a dose of from 0.2 to 20 mg/kg/day to mammals including humans. The composition is administered by intravenous injection either once a day (single administration or continuous administration) or intermittently 1 to 3 times in a week or once in 2 or 3 weeks.

L'invention vise à stabiliser la survie de cellules souches hématopoïétiques transplantées au moyen de lymphocytes activés HLA identiques. A cet effet, on fait proliférer et on active des lymphocytes activés HLA identiques dans des cellules mononucléaires séparées du sang périphérique ou du sang de cordon ombilical. Après séparation et collection, les lymphocytes activés HLA identiques servent de composant principal dans une composition qui vise à stabiliser la survie de cellules souches hématopoïétiques transplantées. Cette composition peut être largement utilisée, par exemple, pour prévenir une survie insuffisante de cellules souches hématopoïétiques transplantées et pour favoriser cette survie. Bien que le dosage de la composition varie en fonction de l'âge, de l'état, etc. du patient, un anticorps humanisé est administré dans une quantité allant de 0,2 à 20 mg/kg/jour aux mammifères, y compris aux humains. Ladite composition est administrée par injection intraveineuse soit une fois par jour (administration unique ou continue), soit une à trois fois par semaine, soit une fois toutes les deux ou trois semaines.

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