C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07H 1/06 (2006.01) C07H 21/04 (2006.01) C12N 9/50 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2600758
An aqueous composition comprising a denaturing agent, a chelator, a buffering agent and a protease for the extraction of nucleic acid from a sample of bodily fluid, such as saliva, such that the extracted nucleic acid is stable for at least fourteen days at room temperature and can be directly utilised in an amplification reaction without further processing. In particular, said composition comprises SDS, Cyclohexanediamine tetraacetate, Tris-HCl and proteinase K. A method and kit for the amplification of DNA directly from a bodily fluid, comprising said composition, is further provided.
La présente invention concerne une composition aqueuse comprenant du SDS, du tétraacétate de cyclohexanediamine, T.pi.s-HCl et la protéinase K destinée à l'extraction d'acide nucléique d'un échantillon de fluide corporel, tel que la salive, l'acide nucléique extrait étant stable pendant au moins quatorze jours à la température ambiante. La composition permet une utilisation directe de l~ADN extraite et stockée dans une réaction d'amplification sans transformation supplémentaire.
Birnboim Chaim H.
Chartier Joanne
Iwasiow Rafal
Jackson Adele
Lem Paul
Dna Genotek Inc.
Osler Hoskin & Harcourt Llp
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