A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
A61K 35/39 (2006.01) A61P 3/10 (2006.01) A61P 5/50 (2006.01) A61K 35/12 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2189052
A method of inducing the proliferation and/or differentiation of human fetal pancreatic cells entails contacting primary cultures of such cells with Hepatocyte Growth Factor/Scatter Factor (HGF/SF), thereby inducing a proliferation of .beta.-epithelial cells, an increase in the number of .beta.-epithelial cells which form islet-like cell clusters, and an increase in insulin production per cell. The method is improved by culturing the cells on an extracellular matrix such as 804G in the presence of HGF/SF, and is further improved by reaggregating thus- treated cells and contacting said cells with an insulin gene upregulating agent such as a poly(ADP-ribose) synthetase inhibitor such as a nicotinamide or benzamide. The method provides increased numbers of functional islet-like cell clusters for transplantation for example, into Type 1 diabetic patients.
Méthode d'induction de la prolifération et/ou de la différentiation de cellules du pancréas du foetus humain consistant à mettre en contact des cultures primaires desdites cellules avec les facteurs de croissance et de dispersion (HGF/SF) ce qui provoque la prolifération des cellules épithéliales .beta. dont l'accroissement donne naissance à des amas cellulaires en îlots et accroît la production d'insuline par cellule. La méthode peut être améliorée en cultivant les cellules sur une matrice extracellulaire telle que la 804G en présence de HGF et de SF, et encore plus, en réagglutinant les cellules ainsi traitées par un agent accroissant l'activité du gène de l'insuline, inhibiteur de la poly (ADP-ribose) synthétase, tel qu'une nicotamide ou une benzamide. La méthode permet de disposer de grandes quantités d'amas cellulaires du genre îlots pour des transplantations, p. ex., chez des patients diabétiques de type 1.
Beattie Gillian M.
Hayek Alberto
Otonkoski Timo Pyry Juhani
Quaranta Vito
Rubin Jeffrey
Department Of He Alth And Human Services United States Of America Represented By The Secretary
Quaranta Vito
Smart & Biggar
The Whittier Institute For Diabetes And Endocrinology
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