Compositions and method of stimulating the proliferation and...

A - Human Necessities – 61 – K

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

A61K 35/39 (2006.01) A61P 3/10 (2006.01) A61P 5/50 (2006.01) A61K 35/12 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2189052

A method of inducing the proliferation and/or differentiation of human fetal pancreatic cells entails contacting primary cultures of such cells with Hepatocyte Growth Factor/Scatter Factor (HGF/SF), thereby inducing a proliferation of .beta.-epithelial cells, an increase in the number of .beta.-epithelial cells which form islet-like cell clusters, and an increase in insulin production per cell. The method is improved by culturing the cells on an extracellular matrix such as 804G in the presence of HGF/SF, and is further improved by reaggregating thus- treated cells and contacting said cells with an insulin gene upregulating agent such as a poly(ADP-ribose) synthetase inhibitor such as a nicotinamide or benzamide. The method provides increased numbers of functional islet-like cell clusters for transplantation for example, into Type 1 diabetic patients.

Méthode d'induction de la prolifération et/ou de la différentiation de cellules du pancréas du foetus humain consistant à mettre en contact des cultures primaires desdites cellules avec les facteurs de croissance et de dispersion (HGF/SF) ce qui provoque la prolifération des cellules épithéliales .beta. dont l'accroissement donne naissance à des amas cellulaires en îlots et accroît la production d'insuline par cellule. La méthode peut être améliorée en cultivant les cellules sur une matrice extracellulaire telle que la 804G en présence de HGF et de SF, et encore plus, en réagglutinant les cellules ainsi traitées par un agent accroissant l'activité du gène de l'insuline, inhibiteur de la poly (ADP-ribose) synthétase, tel qu'une nicotamide ou une benzamide. La méthode permet de disposer de grandes quantités d'amas cellulaires du genre îlots pour des transplantations, p. ex., chez des patients diabétiques de type 1.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Compositions and method of stimulating the proliferation and... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Compositions and method of stimulating the proliferation and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions and method of stimulating the proliferation and... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1518857

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.