Compositions and methods comprising plasmid dna for wound...

A - Human Necessities – 61 – L

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A61L 26/00 (2006.01) A61K 48/00 (2006.01) A61L 24/00 (2006.01)

Patent

CA 2410000

The present invention relates to a method of preparing a composition for wound healing, and for repairing and regenerating human and animal tissue, said method comprising the following steps: a) providing a plasmid DNA in substantially pure form, which encodes a gene that has a positive effect on the progression of the regeneration of the tissue, b) providing a component/components of a self-hardening bio-polymer, and c) providing a cell suspension with cells which promote regeneration, characterized in that components (a), (b) and (c) are incubated with each other simultaneously or successively so that the plasmid and the cell suspension are obtained homogenously distributed in one of the biopolymer components.

L'invention concerne un procédé de préparation d'une composition pour soigner des blessures, ainsi que pour réparer et pour régénérer du tissu humain et animal, ledit procédé comprenant les opérations suivantes : a) fournir un ADN plasmidique sous forme sensiblement pure, codant un gène qui a un effet positif sur l'évolution de la régénération du tissu, b) fournir un/des composant(s) d'un biopolymère autodurcissant, et c) fournir une suspension de cellules promotrices de régénération. L'invention est caractérisée en ce que les composants de (a), (b) et (c) sont incubés ensemble, simultanément ou successivement, de sorte que le plasmide et la suspension de cellules obtenus sont distribués de manière homogène dans un des composants biopolymères.

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