Compositions and methods for assaying subcellular conditions...

G - Physics – 01 – N

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G01N 33/50 (2006.01) C12N 15/72 (2006.01) C12Q 1/02 (2006.01) C12Q 1/04 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2375542

The invention provides compositions and methods for monitoring subcellular compartments such as organelles by energy transfer techniques that do not require specific intermolecular affinity binding events between energy transfer donor and energy transfer acceptor molecules. Provided are methods for assaying cellular membrane potential, including mitochondrial membrane potential, by energy transfer methodologies including fluorescence resonance energy transfer (FRET). Diagnostic and drug screening assays are also provided.

L'invention concerne des compositions et des procédés de surveillance de compartiments infracellulaires, tels que les organites, au moyen de techniques de transfert d'énergie ne nécessitant pas d'événements spécifiques de liaison intermoléculaires par affinité entre des molécules donneuses de transfert d'énergie et des molécules receveuses de transfert d'énergie. L'invention concerne également des procédés d'analyse du potentiel de membranes cellulaires, dont le potentiel de membranes mitochondriales, par des techniques de transfert d'énergie comprenant le transfert d'énergie de résonance de fluorescence (FRET). L'invention concerne en outre des méthodes diagnostiques et des tests de criblage de médicaments.

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