Compositions and methods for controlling insects related to...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) C12Q 1/18 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01) C07K 14/705 (2006.01) C12N 15/12 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2563886

A screening method for identifying compounds that are effective insect control agents includes providing cells expressing an octopamine receptor, adding the compounds to the cells, and measuring the effects of the compounds and compositions. The effects of the compounds may be determined by measuring the binding affinity of the compounds to the octopamine receptor or measuring the change in intracellular cAMP or Ca2+ levels.

L'invention concerne une méthode de criblage permettant d'identifier des composés constituant des agents efficaces de lutte contre les insectes, consistant à utiliser des cellules exprimant un récepteur de l'octopamine, à ajouter les composés aux cellules, et à mesurer les effets des composés et des compositions. Les effets des composés peuvent être déterminés par mesure de l'affinité de liaison des composés au récepteur de l'octopamine ou par mesure du changement des niveaux de cAMP ou Ca2+ intracellulaires.

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