Compositions and methods for detecting and quantifying gene...

Details Compositions and methods for detecting and quantifying gene... Compositions and methods for detecting and quantifying gene...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) B01J 19/00 (2006.01) C03C 17/34 (2006.01) G01N 33/543 (2006.01) G01N 33/552 (2006.01)

Patent

CA 2402525

Compositions and methods for improving detection sensitivity in nucleic acid microarray analysis are disclosed, including methods of purifying nucleic acids, methods of synthesizing fluorescent DNA probes, methods of hybridization, and methods of activating a substrate for target molecule attachment are disclosed.

Cette invention concerne des compositions et des méthodes qui permettent d'améliorer la sensibilité de détection d'une analyse de micro-échantillons d'acides nucléiques. Il s'agit notamment de méthodes de purification d'acides nucléiques, de synthèse de sondes fluorescentes d'ADN, d'hybridation et d'activation d'un substrat pour l'attachement ciblé d'une molécule.

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