Compositions and methods for detecting certain flaviviruses,...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2520538

The present invention provides rapid and accurate methods, primers, probes and kits for identifying the presence of a certain flaviviruses in a sample. Flaviviruses that can be detected include members of the Japanese encephalitis virus serogroup, Dengue virus, St. Louis encephalitis virus, Montana myotis leukoencephalitis virus, Modoc virus, ad Yellow Fever virus. The primers and probes of the invention can hybridize to regions in the 3' untranslated region of the viral genomes to be detected.

La présente invention concerne des procédés rapides et précis, des amorces, des sondes et des nécessaires permettant d'identifier la présence de certains flavivirus dans un échantillon. Les flavivirus pouvant être détectés comprennent des membres du sérogroupe du virus de l'encéphalite japonaise, le virus de la dengue, le virus de l'encéphalite de Saint-Louis, le virus de la leuco-encéphalite des myotis du Montana, le virus du Modoc et le virus de la fièvre jaune. Les amorces et les sondes selon l'invention peuvent s'hybrider à des régions dans la région non traduite 3' des génomes viraux à détecter.

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