Compositions and methods for detecting protein modification...

G - Physics – 01 – N

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G01N 33/543 (2006.01) C12Q 1/00 (2006.01) C12Q 1/48 (2006.01) G01N 33/573 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2383945

This invention relates generally to the field of protein modification, e.g., post-translational modification. In particular, the invention provides a method for detecting protein modification profile in a sample, which method comprises: a) contacting a sample containing or suspected of containing a target protein with a capture molecule, or a plurality of capture molecules, immobilized on a solid support, said capture molecule is capable of specifically binding to said target protein, whereby said target protein is immobilized on said solid support; and b) assessing modification status and/or identity of said immobilized target protein. Kits and arrays useful for detecting protein modification are also provided. Arrays, kits and methods useful for detecting enzymatic activities, especially protein modification enzymatic activities, are further provided.

De manière générale, l'invention concerne le domaine de la modification des protéines, telle que la modification post-translationnelle. Plus particulièrement, l'invention concerne un procédé permettant de détecter un profil de modification de protéine dans un échantillon. Ce procédé consiste (a) à mettre un échantillon contenant ou pouvant contenir une protéine cible au contact d'une molécule de capture, ou d'une pluralité de molécules de capture, immobilisée sur un support solide, cette molécule de capture étant capable de se lier spécifiquement à cette protéine cible, laquelle est immobilisée sur ce support solide, et (b) à déterminer l'identité et/ou le statut de modification de cette protéine cible immobilisée. L'invention concerne également des trousses et des jeux ordonnés d'échantillons destinés à la détection d'une modification de protéine. Elle se rapporte en outre à des jeux ordonnés d'échantillons, des trousses et des procédés permettant de détecter des activités enzymatiques, et notamment des activités enzymatiques liées à une modification de protéine.

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