C - Chemistry – Metallurgy – 07 – F
Patent
C - Chemistry, Metallurgy
07
F
C07F 5/02 (2006.01) C07D 239/72 (2006.01) C07D 311/82 (2006.01) C07D 495/04 (2006.01) C07D 498/22 (2006.01) C07F 5/00 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2483868
The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
L'invention concerne des composés à liaison phosphate utilisés pour lier, détecter et isoler des molécules cibles phosphorylées, et notamment, pour identifier des molécules cibles interagissant avec des molécules cibles phosphorylées ou à phosphoryler. Une solution de liaison prévue comprend un composé à liaison phosphate, un acide et un ion métallique, ledit ion métallique interagissant simultanément avec un groupe phosphate exposé sur une molécule cible, et la fraction de chélation métallique du composé à liaison phosphate formant un pont entre ledit composé à liaison phosphate et une molécule cible phosphorylée produisant un complexe ternaire. La solution de l'invention est utilisée pour lier et immobiliser des molécules cibles phosphorylées, pour isoler les molécules cibles phosphorylées d'un mélange de complexe et faciliter une analyse protéomique dans laquelle des substrats de kinase et de phosphatase et des enzymes peuvent être identifiés.
Agnew Brian
Beechem Joseph
Gee Kyle
Haugland Richard
Liu Jixiang
Mbm Intellectual Property Law Llp
Molecular Probes Inc.
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