Compositions and methods for detection of propionibacterium...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C40B 40/06 (2006.01)

Patent

CA 2715396

Methods for amplifying and detecting Propionibacterium acnes nucleic acid by targeting specific sequences in 16S rRNA, 23S rRNA, or DNA encoding 16S rRNA or 23S rRNA are disclosed. Nucleic acid oligonucleotide sequence compositions specific for P. acnes nucleic acid sequences in 16S or 23S rRNA or DNA encoding 16S or 23S rRNA sequences are disclosed, which are useful for amplification oligonucletides, capture probes in sample preparation, and probes for detection of P. acnes nucleic acid sequences.

La présente invention concerne des procédés d'amplification et de détection d'acide nucléique de Propionibacterium acnes par ciblage de séquences spécifiques dans l'ARN ribosomal 16S ou 23S, ou l'ADN codant ces ARN. L'invention concerne également des compositions de séquences d'oligonucléotides d'acide nucléique qui, d'une part sont spécifiques des séquences d'acide nucléique de Propionibacterium acnes présentes dans l'ARN ribosomal 16S ou 23S ou dans l'ADN codant les séquences d'ARN ribosomal 16S ou 23S, et qui, d'autre part conviennent particulièrement comme oligonucléotides d'amplification, comme sondes de capture pour la préparation d'échantillons, et comme sondes pour la détection de séquences d'acide nucléique de Propionibacterium acnes.

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