Compositions and methods for determining the replication...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/70 (2006.01)

Patent

CA 2555140

This invention relates to methods for predicting replication capacity of a virus based on genotype and identifying targets for antiviral therapy by identifying mutations associated with altered replication capacity. The methods are useful, for example, for identifying previously unknown interactions among viral molecules or between viral molecules and host cell molecules that are essential to viral infection and/or replication. By identifying such interactions, novel targets for antiviral therapy can be identified. In another aspect, the invention provides a method for determining that an HIV has an altered replication capacity. In certain embodiments, the method comprises detecting a mutation in a codon of gag that is selected from the group consisting of 437, 439, 441, 442, 454, 478, 479, and 484. In certain embodiments, the mutation is selected from the group consisting of I437L, P439S, E454V, P478L, and I479K. In certain embodiments, the mutation is in a codon of gag that is selected from the group consisting of 418, 456, 456, 453, 418, 483, 481, 465, 429, 484, 481, 483, 484, 465, 454, 442, 479, 418, 479, and 486.

La présente invention se rapporte à des procédé permettant de prédire la capacité de réplication d'un virus sur la base de son génotype, et d'identifier des cibles pour la thérapie antivirale, par l'identification de mutations associées à une capacité de réplication altérée. Les procédés selon l'invention sont utiles, par exemple, pour identifier des interactions précédemment inconnues entre molécules virales ou entre des molécules virales et des molécules de cellules hôtes, qui sont essentielles à l'infection et/ou à la réplication virales. L'identification de telles interactions permet d'identifier de nouvelles cibles pour la thérapie antivirale. Dans un autre aspect, l'invention a trait à un procédé permettant de déterminer qu'un VIH présente une capacité de réplication altérée. Dans certains modes de réalisation, le procédé selon l'invention consiste à détecter une mutation dans un codon degag qui est sélectionné parmi les codons 437, 439, 441, 442, 454, 478, 479 et 484. Dans certains modes de réalisation, la mutation est sélectionnée parmi I437L, P439S, E454V, P478L et I479K. Dans certains modes de réalisation, la mutation se situe dans un codon de gag qui est sélectionné parmi les codons 418, 456, 456, 453, 418, 483, 481, 465, 429, 484, 481, 483, 484, 465, 454, 442, 479, 418, 479 et 486.

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