Compositions and methods for enhanced expression of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/63 (2006.01) C12N 15/00 (2006.01) C12N 15/86 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2526120

Vector constructs for expression of two or more functional proteins or polypeptides under operative control of a single promoter and methods of making and using the same are described. The vectors comprise a self- processing cleavage site between each respective protein or polypeptide coding sequence. The vector constructs include the coding sequence for a self- processing cleavage site and may further include an additional proteolytic cleavage sequence which provides a means to remove the self processing peptide sequence from expressed protein(s) or polypeptide(s). The vector constructs find utility in methods for enhanced production of biologically active proteins and polypeptides in vitro and in vivo.

L'invention concerne des constructions vectorielles pour une expression d'au moins deux protéines ou deux polypeptides fonctionnels, sous le contrôle fonctionnel d'un promoteur unique. L'invention concerne également des méthodes de fabrication et d'utilisation de ces constructions. Les vecteurs de l'invention comprennent un site de clivage autocatalytique, situé entre chaque séquence de codage protéinique ou polypeptidique correspondante. Les constructions vectorielles comprennent la séquence de codage pour un site de clivage autocatalytique et peuvent également comprendre une séquence de clivage protéolytique supplémentaire constituant un moyen pour retirer la séquence peptidique d'autotraitement de la protéine ou des protéines, ou du polypeptide ou des polypeptides exprimé(s). Les constructions vectorielles s'avèrent utiles dans des méthodes destinées à améliorer la production de protéines et de polypeptides biologiquement actifs, <i>in vitro</i> et <i>in vivo</i>.

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