Compositions and methods for expression of a heterologous...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01)

Patent

CA 2736244

The present invention provides compositions and methods for targeting polypeptides to the chloroplasts of higher plants. Compositions include expression cassettes having a nucleotide sequence encoding a chloroplast targeting peptide (CTP) operably linked to a nucleotide sequence of interest, wherein the CTP is derived from Chlamydomonas sp. Plant transformation vectors, plants and plant cells having the CTP sequences are also encompassed, as well as variants and fragments of the CTP sequences. Methods for expressing a heterologous nucleotide sequence in a plant using the CTP sequences disclosed herein are also provided.

La présente invention a pour objet des compositions et des procédés pour le ciblage de polypeptides sur les chloroplastes des plantes supérieures. Les compositions comprennent des cassettes dexpression ayant une séquence de nucléotides codant un peptide de ciblage de chloroplaste (CTP) lié de façon fonctionnelle à une séquence de nucléotides dintérêt, le CTP étant dérivé de Chlamydomonas sp. Des vecteurs de transformation de plante, des plantes et des cellules de plante ayant les séquences de CTP sont également englobés, ainsi que des variantes et des fragments des séquences de CTP. La présente invention concerne également des procédés pour lexpression dune séquence de nucléotides hétérologues chez une plante au moyen des séquences de CTP décrites ici.

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