Compositions and methods for extracting a nucleic acid

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01)

Patent

CA 2430138

The present invention provides aqueous compositions comprising sodium metasilicate and an ether and methods of using the compositions to extract a nucleic acid from a cell, virus or other source. The extracted nucleic acids can be used for a variety of purposes, including as a source of template DNA for a polymerase chain reaction.

La présente invention concerne des compositions aqueuses qui comprennent du métasilicate de sodium et un éther et des techniques d'utilisation de ces compositions de façon à extraire un acide nucléique d'une cellule, d'un virus ou d'une autre source. On peut utiliser ces acides nucléiques extraits à diverses fins, notamment comme source de matrice d'ADN pour une réaction en chaîne de la polymérase.

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