Compositions and methods for generating chimeric...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/13 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C07K 17/00 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2455237

The present invention provides a technique for specific assembly of monomericpolypeptides to form a heteromultimer. This technique is particularly useful for generating a genetically diverse repertoire of heteromultimers such as antigen-binding units. The invention also provides both non-single-chain and single-chain antigen-binding units that are assembled by the technique described herein. The present invention also provides recombinant polynucleotides, vectors, host cells, and kits for producing the subjectantigen-binding units. Further provided by the invention are methods of using the subject antigen-binding units.

L'invention concerne une technique consistant à former un hétéromultimère à partir d'un ensemble spécifique de polypeptides monomères. Cette technique est spécialement indiquée pour la génération d'un répertoire génétiquement différent d'hétéromultimères, dont des unités de liaison d'antigène. L'invention concerne également des unités de liaison d'antigène non simple chaîne et simple chaîne assemblées par la technique précitée. L'invention concerne de plus des nucléotides recombinants, des vecteurs, des cellules hôtes et des kits de production d'unité de liaison d'antigène sujet. Font également l'objet de cette invention des procédés d'utilisation des unités de liaison d'antigène sujet.

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