Compositions and methods for generating monoclonal...

C - Chemistry – Metallurgy – 07 – K

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C07K 16/00 (2006.01) A61K 39/39 (2006.01) C07K 14/705 (2006.01) C07K 16/28 (2006.01) C12N 5/20 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2352471

The present invention provides a method for generating a population of monoclonal antibodies capable of binding to antigens representative of a particular cell type. The method is unique in that it minimizes the generation of non-representative antibodies cross-reacting with proteins not present in a particular cell type; it also maximizes the preservation of intact antigens, especially surface antigens, for production of a plurality of monoclonal antibodies that bind to the native antigens. The present invention also includes a method of determining the combination of cell surface antigens present on a specific cell type. Further provided by the invention are hybridomas, populations of monoclonal antibodies produced by the hybridomas of the present invention.

Cette invention concerne un procédé permettant de produire une population d'anticorps capables de se lier à des antigènes représentatifs d'un type particulier de cellule. L'originalité du procédé réside en ce qu'il réduit au minimum la production d'anticorps non représentatifs ayant des réactions croisées avec des protéines qui ne sont pas présentes dans un type particulier de cellule, et qu'il maximise par ailleurs la conservation d'antigènes intacts, en particulier d'antigènes d'enveloppe, en vue de la production d'anticorps monoclonaux qui se lient avec les antigènes natifs. Cette invention concerne également un procédé permettant de déterminer la combinaison d'antigènes d'enveloppe présents sur un type cellulaire déterminé. L'invention porte enfin sur des hybridomes et des populations d'antigènes monoclonaux produits par ces hybridomes.

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