Compositions and methods for genetic analysis

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2366107

The present invention relates to genetic mapping of complex quantitative and qualitative traits. This invention more particularly relates to compositions and methods to identify identical DNA fragments from two different DNA sources. The method allows the amplification of the DNA's, their labelling, and the separation of perfectly matched DNA's from imperfectly matched DNA's or from DNA's formed through hybridisation from the same source (e.g., homohybrids). The invention can be used to identify genes or gene mutations, which are relevant to pathological conditions or particular traits.

La présente invention concerne la cartographie génétique de traits complexes quantitatifs et qualitatifs. Plus particulièrement, l'invention concerne des compositions et des méthodes d'identification de fragments d'ADN identiques provenant de deux sources d'ADN différentes. En outre, cette méthode permet l'amplification des ADN, leur marquage, et la séparation d'ADN parfaitement appariés des ADN mal appariés ou d'ADN formés par hybridation dans la même source (par exemple, des homohybrides). En l'occurrence, on peut utiliser cette invention pour identifier les gènes, ou les mutations géniques, relatifs à des états pathologiques ou à des traits particuliers.

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