Compositions and methods for growth of embryonic stem cells

Details Compositions and methods for growth of embryonic stem cells Compositions and methods for growth of embryonic stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01)

Patent

CA 2558486

The present invention relates to methods, compositions, and kits for maintenance of the undifferentiated state and/or pluripotency in stem cells, including embryonic stem (ES) cells. It also relates to stem cells that have been maintained in an undifferentiated state. The present invention provides these aspects using culture medium enriched with a TGF.beta. family member protein, an FGF family member protein, and/or nicotinamide, without using fibroblast feeder layers, conditioned media, or leukemia inhibitory factor.

La présente invention concerne des procédés, des compositions et des kits pour maintenir l'état non différencié et/ou la pluripotence dans des cellules souches, notamment des cellules souches embryonnaires (ES). Elle concerne également des cellules souches qui ont été maintenues dans un état non différencié. Cette invention met en oeuvre un milieu de culture enrichi en protéine de la famille TGF.beta., en protéine de la famille FGF et/ou en nicotinamide, sans utiliser de couches nourricières de fibroblastes, de milieu conditionné ou de facteur LIF (leukemia inhibitory factor).

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