Compositions and methods for growth of pluripotent cells

Details Compositions and methods for growth of pluripotent cells Compositions and methods for growth of pluripotent cells

C - Chemistry, Metallurgy

07

K

C07K 14/78 (2006.01) C07K 1/04 (2006.01) C07K 7/00 (2006.01) C07K 17/00 (2006.01) C07K 17/14 (2006.01) C12M 3/00 (2006.01) C12N 5/02 (2006.01) C12N 11/00 (2006.01) C12N 11/06 (2006.01) C12N 15/12 (2006.01) C40B 80/00 (2006.01)

Patent

CA 2616863

A method of propagating embryonic stem (ES) cells in an undifferentiated state, while maintaining both the pluripotency and the cells normal genotype is disclosed. The method comprises using recombinantly produced protein domains to attach human embryonic stem cells to the surface of a bioreactor. The ES cells are supplied with nutrients while they held in place by the recombinantly produced protein domains which may be chosen from Laminin G domain, Fibronectin domain 2, Fibronectin domain 3, Nidogen G2 domain, Nidogen G3 domain, Vitronectin somatomedin B domain, and Vitronectin somatomedin C terminal domain. Useful molecules are characterized by a high binding affinity for hES cells and a molecular weight of about 50kDa ~ 20%.

L'invention concerne un procédé de propagation de cellules souches (CS) embryonnaires dans un état indifférencié tout en préservant le caractère pluripotent et le génotype normal des cellules. Le procédé comprend le recours à des domaines de protéine produits par recombinaison pour fixer des cellules souches embryonnaires humaines à la surface d'un bioréacteur. Les cellules CS reçoivent des nutriments pendant qu'elles sont tenus en place par les domaines de protéine produits par recombinaison qui peuvent avoir été choisis du domaine G de la laminine, des domaines 2, 3 de la fibronectine, des domaines G2, G3 du nidogène, du domaine somatomédine B de la vitronectine, et du domaine terminal somatomédine C de la vitronectine. Les molécules utiles se caractérisent par une affinité de liaison élevée pour les cellules CSh et un poids moléculaire d'environ 50kDa ± 20 %.

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