Compositions and methods for improved protein production

Details Compositions and methods for improved protein production Compositions and methods for improved protein production

C - Chemistry, Metallurgy

12

N

C12N 15/80 (2006.01)

Patent

CA 2661808

The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p,8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p,8k, 7E, 9G, 8Q and 203, which are presented in Figure 1, and are SEQ ID NOS.: 1 -6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.

L'invention concerne l'identification de nouvelles séquences d'acide nucléique, soit, ici, 7p, 8k, 7E, 9G, 8Q et 203, dans une cellule hôte qui assure la production de protéines. L'invention concerne également des cellules hôtes qui ont une mutation ou une délétion partielle ou totale du gène codant 7p, 8k, 7E, 9G, 8Q et 203, qui sont présentés en figure 1 et qui sont SEQ ID NOS.:1-6, respectivement. L'invention concerne en outre des cellules hôtes comprenant un acide nucléique codant une protéine hétérologue recherchée, par exemple un enzyme.

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