C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/12 (2006.01) C07K 19/00 (2006.01) C12N 9/16 (2006.01) C12N 9/20 (2006.01) C12N 9/22 (2006.01) C12N 9/24 (2006.01) C12N 9/48 (2006.01) C12N 9/99 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2614444
Compositions and methods are provided that improve the specificity and efficiency of nucleic acid amplification. A binding partner may be bound to a DNA polymerase enzyme where the binding partner substantially inhibits the activity of the polymerase. A second enzyme modifies the binding partner in a manner that relieves the inhibition of the polymerase activity. The activity of the second enzyme may be inhibited in a temperature-sensitive manner such that the second enzyme is active only at elevated temperatures. As a consequence, the polymerase enzyme also is active only when the temperature is elevated.
L'invention porte sur des compositions et des méthodes améliorant la spécificité et l'efficacité de l'amplification d'acides nucléiques. Un partenaire de liaison peut se fixer à une ADN polymérase lorsqu'il en inhibe quasiment l'activité, tandis qu'une deuxième enzyme modifie le partenaire de liaison de manière à faire cesser l'inhibition de l'activité de la polymérase. L'activité de la deuxième enzyme peut être inhibée par un phénomène fonction de la température, tel que la deuxième enzyme ne soit active qu'à des températures élevées. Par conséquent, la polymérase n'est également active que quand la température est élevée.
Rashtchian Ayoub
Schuster David M.
Mbm Intellectual Property Law Llp
Quanta Biosciences Inc.
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