Compositions and methods for inducing the activation of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2632263

The present invention provides methods for inducing the maturation of immature dendritic cells (DC) and for activating those cells without the use of a dendritic cell maturation agent. The activated DC can be used for inducing an antigen specific T cell response. Methods of the invention can also comprise the addition of a directional maturation agent, such as interferon gamma, to induce a Th-I and/or Th-2 bias in the response obtained. The present invention also provides dendritic cell populations useful for activating and for inducing antigen specific T cells. Similarly, activated antigen specific T cell populations, and methods of making the same are provided.

Cette invention concerne des procédés permettant d'induire la maturation de cellules dendritiques (DC) immatures et d'activer ces cellules sans l'aide d'un agent de maturation de cellules dendritiques. Les cellules dendritiques activées peuvent être utilisées pour induire une réponse des lymphocytes T spécifiques de l'antigène. Des procédés de cette invention peuvent également consister à ajouter un agent de maturation directionnel, tel qu'un interféron gamma, pour induire une polarisation de Th-1 et/ou Th2 dans la réponse obtenue. Cette invention concerne également des populations de cellules dendritiques utilisées pour activer et pour induire des lymphocytes T spécifiques de l'antigène, de même que des populations de lymphocytes T spécifiques de l'antigène activées et des procédés de production correspondants.

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