Compositions and methods for inhibiting light-induced...

G - Physics – 01 – N

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G01N 33/58 (2006.01) G01N 33/533 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2286183

Disclosed is a fluorescent conjugate comprising a biological reagent, a fluorescent molecule, and a means for impeding phototoxic degradation of the biological reagent. The impeding means can be a cross- linking substance having a long molecular distance, whereby the cross-linking substance links the fluorescent molecule and the biological reagent; a quencher of singlet oxygen; a quencher of a free radical; or a combination thereof. Also disclosed is a solution comprising a fluorescent conjugate of a biological reagent and a fluorescent molecule together with an oxygen depleting system.

L'invention porte sur un conjugué fluorescent comprenant un réactif biologique, une molécule fluorescente et un moyen permettant de limiter la dégradation photoxique du réactif biologique. Ce moyen de limitation peut être une substance de réticulation présentant une longue distance moléculaire, la substance de réticulation liant la molécule fluorescente et le réactif biologique; un extincteur de luminescence d'oxygène singulet; un extincteur de luminescence d'un radical libre; ou une combinaison de ceux-ci. L'invention porte également sur une solution comprenant un conjugué fluorescent d'un réactif biologique et une molécule fluorescente, ainsi qu'un système d'épuisement d'oxygène.

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