C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/70 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2374515
The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.
De manière générale, la présente invention concerne des compositions, des trousses et des méthodes destinées au marquage de molécules d'acide nucléique à l'aide de transcriptases inverses, de préférence à l'aide de transcriptases inverses à plusieurs sous-unités, telles que les transcriptases inverses ASLV. Plus particulièrement, l'invention concerne des méthodes, des trousses et des compositions destinées au marquage fluorescent de molécules d'acide nucléique au cours de la synthèse d'acide nucléique. Les molécules d'acide nucléique marquées selon l'invention sont notamment utilisées comme sondes marquées pour la détection d'acide nucléique et les diagnostics.
Gruber Christian E.
Shih Po-Jen
Invitrogen Corporation
Mbm & Co.
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