Compositions and methods for measuring analyte concentrations

G - Physics – 01 – N

Patent

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G01N 33/542 (2006.01)

Patent

CA 2546949

The current invention relates to fusion proteins comprising at least one functional periplasmic binding protein, at least one labeling moiety and at least one fluorescent protein. In one embodiment, the periplasmic binding protein is a functional glucose-galactose binding protein (GGBP). The invention also relates to methods for quantifying an analyte, for example glucose, in a cell or tissue comprising administering a composition comprising a fluorescent periplasmic binding fusion protein portion to the cell or tissue, and measuring the fluorescence of the fluorescent periplasmic binding fusion protein.

L'invention concerne des protéines hybrides comprenant au moins une protéine de liaison périplasmique, au moins une fraction de marquage et au moins une protéine fluorescente. Dans un mode de réalisation, la protéine de liaison périplasmique est une protéine de liaison glucose-galactose fonctionnelle (GGBP). L'invention concerne également des méthodes pour quantifier un analyte, par exemple du glucose, dans une cellule ou dans un tissu. Ces méthodes consistent à administrer une composition comprenant une protéine hybride de liaison périplasmique fluorescente à la cellule ou au tissu susmentionné, et à mesurer la fluorescence de cette protéine hybride de liaison périplasmique fluorescente.

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