Compositions and methods for modulating cytokine release in...

A - Human Necessities – 61 – K

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A61K 31/70 (2006.01) A61K 31/00 (2006.01) A61K 31/436 (2006.01) A61K 38/13 (2006.01) A61K 38/43 (2006.01) A61K 41/00 (2006.01)

Patent

CA 2319567

A previously unknown mechanism for cytokine release in response to genotoxic agents, as well as therapeutic and diagnostic procedures and techniques which are based on that mechanism, are disclosed. The mechanism involves the recognition of damaged DNA by DNA-protein kinases and the subsequent phosphorylation of substrates that leads to cytokine release. Methods to modulate the activity of DNA-protein kinases and thus the release of cytokines in response to genotoxic agents are described. Assays for DNA-protein kinase activity which can be used to monitor such modulation are also disclosed.

L'invention concerne un mécanisme inconnu auparavant de libération de cytokine en réponse à des agents génotoxiques, ainsi que des procédés et techniques thérapeutiques et diagnostiques sur la base de ce mécanisme. Le mécanisme consiste en la reconnaissance d'ADN endommagé par des protéines d'ADN kinases et la phosphorylation ultérieure de substrats entraînant une libération de cytokine. L'invention concerne également des méthodes de modulation de l'activité de protéines d'ADN kinases et donc de la libération de cytokines en réponse à des agents génotoxiques. L'invention concerne enfin des dosages de l'activité de protéines d'ADN kinases, pouvant s'utiliser pour contrôler une telle modulation.

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