Compositions and methods for modulation of cell migration

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/705 (2006.01) A61K 38/08 (2006.01) A61K 38/17 (2006.01) A61K 49/00 (2006.01) A61P 35/04 (2006.01) C07K 7/06 (2006.01) C12Q 1/00 (2006.01) C40B 30/04 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2754482

A peptide includes SEQ ID NO:3, substitution and addition variants thereof which maintain the ability to activate CD44. A complex includes this peptide or an A6 polypeptide with a CD44 polypeptide. An isolated polypeptide includes the Link region sequence of human CD44, functionally active fragments thereof, substitution variants, and addition variants. A method of treating a disease characterized by aberrant cell migration and/or invasion includes administering to a subject an effective amount of the peptide of SEQ ID NO:3 or an A6 polypeptide to bind to a CD44 polypeptide and modulate signal transduction activity for a sufficient period of time to treat the disease. Other methods include using the peptide of SEQ ID NO:3 or an A6 polypeptide for diagnosing, identifying a subpopulation of subjects responsive to treatment, and screening for compounds that bind a CD44 polypeptide.

L'invention porte sur un peptide qui comprend SEQ ID N°:3, des variants par substitution et addition de celui-ci qui conservent la capacité d'activer CD44. Un complexe comprend ce peptide ou un polypeptide A6 avec un polypeptide CD44. Un polypeptide isolé comprend la séquence de région de liaison de CD44 humain, des fragments fonctionnellement actifs de celle-ci, des variants par substitution et des variants par addition. Une méthode de traitement d'une maladie caractérisée par une migration et/ou une invasion cellulaire aberrante consiste à administrer à un sujet une quantité efficace du peptide de SEQ ID N°:3 ou un polypeptide A6 en vue d'une liaison avec un polypeptide CD44 et d'une modulation de l'activité de transduction de signal pendant une période de temps suffisante pour traiter la maladie. D'autres méthodes consistent à utiliser le peptide de SEQ ID N°:3 ou un polypeptide A6 pour le diagnostic, l'identification d'une sous-population de sujets sensibles au traitement, et le criblage de composés qui se lient à un polypeptide CD44.

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