Compositions and methods for multiplex analysis of...

Details Compositions and methods for multiplex analysis of... Compositions and methods for multiplex analysis of...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2516299

Provided herein are compositions and methods for the multiplex analysis and/or detection of polynucleotides having one or more distinguishable target sequences. The methods employ signal-quencher probe pairs having specific relative differential thermal melting temperatures that permit the detection of one or more target sequences on one or more polynucleotides.

Cette invention se rapporte à des compositions et à des procédés pour l'analyse et/ou la détection multiplex de polynucléotides ayant une ou plusieurs séquences cibles identifiables. Ces procédés utilisent des paires de sondes extincteurs de signal ayant des températures de fusion thermique différentielles relatives spécifiques qui permettent la détection d'une ou de plusieurs séquences cibles sur un ou plusieurs polynucléotides.

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