Compositions and methods for non-parenteral delivery of...

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/04 (2006.01) A61K 9/00 (2006.01) A61K 9/107 (2006.01) A61K 31/7088 (2006.01) A61K 47/44 (2006.01) A61K 48/00 (2006.01) A61P 1/04 (2006.01) C12N 15/11 (2006.01) A61K 38/00 (2006.01) A61K 47/12 (2006.01) A61K 47/28 (2006.01)

Patent

CA 2329130

The present invention relates to compositions and methods which enhance the local and systemic uptake and delivery of oligonucleotides and nucleic acids via non-parenteral routes of administration. Pharmaceutical compositions comprising oligonucleotides disclosed herein include, for systemic delivery, emulsion and microemulsion formulations for a variety of applications and oral dosage formulations. It has also suprisingly been discovered that oligonucleotides may be locally delivered to colonic sites be rectal enemas and suppositories in simple solutions, e.g., neat or in saline. Such pharmaceutical compositions of olignonucleotides may further include one or more penetration enhancers for the transport of oligonucleotides and other nucleic acids across mucosal membranes. The compositions and methods of the invention are utilized to effect the oral, buccal, rectal or vaginal administration of an antisense oligonucleotide to an animal in order to modulate the expression of a gene in the animal for investigative, therapeutic, palliative or prophylactic purposes.

L'invention concerne des compositions et des procédés augmentant l'absorption et la libération locales et systémiques des oligonucléotides et des acides nucléiques, via des voies d'administration non parentérales. Les compositions pharmaceutiques renfermant des oligonucléotides présentement décrites, comprennent, pour l'administration systémique, des formulations en émulsion et micro-émulsion pour une variété d'applications et des formulations de dosage par voie orale. On a constaté avec surprise que des oligonucléotides peuvent être administrés localement sur des sites coliques par lavement rectal et suppositoires dans des solutions simples, par exemple, pures ou salines. De telles compositions pharmaceutiques d'oligonucléotides peuvent renfermer en outre un ou plusieurs activateurs de pénétration pour le transfert des oligonucléotides et autres acides nucléiques à travers les membranes muqueuses. Les compositions et les procédés de l'invention sont utilisés pour l'administration par voie orale, buccale, rectale ou vaginale d'un oligonucléotide antisens à un animal, en vue de moduler l'expression d'un gène dans l'animal à des fins de recherches, thérapeutiques, palliatives ou prophylactiques.

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