Compositions and methods for nucleic acid or polypeptide...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2408728

The present invention relates to compositions and methods for nucleic acid analyses. More particularly, this invention provides compositions and methods for differential gene expression analyses on nucleic acid arrays. This invention discloses more preferably differential gene expression analyses on nucleic acid arrays using nucleic acid samples having distinct radioactive labels. Even more particularly, this invention relates to compositions and methods for nucleic acid analysis, comprising contacting at least two differently radiolabelled nucleic acid samples on a nucleic acid array, and detecting (or comparing or quantifying) hybrids formed between the nucleic acids of the samples and the nucleic acid array. The present invention can be used to detect or monitor gene expression or to compare gene expression (e.g., differential gene expression screening), for instance, and is suitable for use in research, diagnostic and many pharmacogenomics applications, for instance.

L'invention concerne des compositions et procédés destinés aux analyses d'acides nucléiques et notamment des compositions et procédés destinés aux analyses d'expression génique différentielle sur des réseaux d'acides nucléiques. L'invention concerne plus précisément des analyses d'expression génique différentielle sur des réseaux d'acides nucléiques utilisant des échantillons d'acides nucléiques qui possèdent des étiquettes radioactives distinctes; elle porte surtout sur des compositions et procédés destinés aux analyses d'acides nucléiques qui consistent à mettre en contact au moins deux échantillons d'acides nucléiques à étiquettes radioactives sur un réseau d'acides nucléiques et détecter (ou comparer et quantifier) des hybrides formés entre les acides nucléiques des échantillons et le réseau d'acides nucléiques. L'invention peut s'utiliser pour détecter ou surveiller l'expression génique ou comparer l'expression génique (par exemple, le criblage d'expression génique différentielle) et convient à la recherche, à la diagnostique et, par exemple, à de nombreuses applications pharmacogénomiques.

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