C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2719747
Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
La présente invention concerne des compositions et des procédés pour le séquençage dacide nucléique qui comprennent des constructions de matrice qui comprennent des parties bicaténaires dans des constructions partiellement ou totalement contiguës, pour permettre la détermination de séquence redondante par lintermédiaire de lun ou des deux parmi le séquençage de brins sens et antisens, et le séquençage itératif de la construction totale plusieurs fois. Des composants de séquence additionnels sont également facultativement inclus dans de telles constructions de matrice. Des procédés sont également proposés pour lutilisation et la préparation de ces constructions ainsi que des compositions de séquençage pour leur application.
Heiner Cheryl
Ma Congcong
Otto Geoff
Travers Kevin
Turner Stephen
Fetherstonhaugh & Co.
Pacific Biosciences Of California Inc.
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