C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2753271
The invention provides methods and compositions for separately denaturing a probe and target in hybridization applications. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
La présente invention concerne des procédés et des compositions permettant de dénaturer séparément une sonde et une cible au cours d'applications d'hybridation. L'invention permet, par exemple, de supprimer l'utilisation de formamide au cours d'applications d'hybridation, ou de moins en dépendre. Les compositions destinées à être utilisées dans le cadre de l'invention incluent une composition aqueuse contenant au moins un solvant aprotique polaire en une quantité efficace pour dénaturer des séquences nucléotidiques double brin.
Dako Denmark A/s
Fetherstonhaugh & Co.
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