Compositions and methods for plant genetic modification

Details Compositions and methods for plant genetic modification Compositions and methods for plant genetic modification

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2389441

Mixed duplex oligonucleotides (MDON) are used to effect site-specific genetic alterations in a target DNA sequence of a plant. The MDON are introduced by electroporation into microspores. Thereafter, plants having a desired genetic alteration are produced by germinating the microspores.

Selon l'invention, des oligonucléotides à double hélice (MDON) sont utilisés pour effectuer des modifications génétiques spécifiques au sites dans une séquence d'ADN cible d'un végétal. Les oligonucléotides à double hélice (MDON) sont introduits par électroporation dans des microspores. Ensuite, les végétaux présentant une modification génétique souhaitée sont produits par germination des microspores.

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