A - Human Necessities – 01 – H
Patent
A - Human Necessities
01
H
A01H 4/00 (2006.01)
Patent
CA 2287942
Improved compositions and methods for transformation and regeneration of plants from embryogenic callus are disclosed that include, for example: use of an intermediate-incubation medium after callus induction to increase the competence of the transformed cells for regeneration; dim light conditions during early phases of selection; use of green callus tissue as a target for microprojectile bombardment; and media with optimized levels of phytohormones and copper concentrations.
L'invention concerne des compositions et des méthodes améliorées, qui permettent de transformer et de régénérer des végétaux à partir de cals embryogènes. Parmi ces méthodes, on trouve l'utilisation d'un milieu d'incubation intermédiaire après induction du cal afin d'augmenter la compétence des cellules transformées à la régénération; l'éclairage faible durant les phases précoces de la sélection; l'utilisation de tissus de cal verts comme cibles pour le bombardement de micro-projectiles; et l'utilisation de milieux ayant une concentration optimisée en phytohormones et en cuivre.
Cho Myeong-Je
Lemaux Peggy G.
Sim & Mcburney
The Regents Of The University Of California
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