Compositions and methods for polynucleotide sequence detection

Details Compositions and methods for polynucleotide sequence detection Compositions and methods for polynucleotide sequence detection

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2516306

The present invention provides compositions, kits, and methods for detecting polynucleotide sequence differences. The method involves amplifying a polynucleotide in the presence of a labeled nucleotide whose incorporation into the amplified product can indicate the presence of a sequence difference within the polynucleotide template. The invention is particularly useful for differentiating two or more closely related polynucleotide sequences, for example, in determining which allele or alleles of a multiallelic organism are present in a target polynucleotide.

Compositions, kits et méthodes de détection de différences dans des séquences polynucléotidiques. Ladite méthode consiste à amplifier un polynucléotide en présence d'un nucléotide marqué dont l'incorporation dans le produit amplifié peut indiquer la présence d'une différence de séquence au sein de la matrice de polynucléotides. La présente invention est particulièrement utile pour différencier deux séquences polynucléotidiques étroitement apparentées ou plus, par exemple en déterminant quel(s) allèle(s) d'un organisme multiallélique est (sont) présent(s) dans un polynucléotide cible.

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